Novel keratinase from Bacillus subtilis S14 exhibiting remarkable dehairing capabilities.
نویسندگان
چکیده
We report the isolation of a keratinolytic-producing Bacillus subtilis strain and the characterization of the exceptional dehairing properties of its subtilisin-like keratinase. This enzyme can be an alternative to sodium sulfide, the major pollutant from tanneries, and may completely replace it. Its unique nonactivity upon collagen enhances its industrial potential.
منابع مشابه
Characterization of the keratinolytic activity of indigenous Bacillus subtilis keratinase
Keratins are water insoluble proteins of our environment. Being extremely resistant to degradation by proteolytic enzymes, keratins are digested mainly by alkali and keratinase enzymes. The aim of present study was to characterize the indigenous keratinase for potentials of keratin-degrading activities. We report the purification and characterization of keratinase from Bacillus subtilis isolate...
متن کاملSCREENING AND CHARACTERIZATION OF KERATINASE FROM Bacillus licheniformis ISOLATED FROM NAMAKKAL POULTRY FARM
Keratin are insoluble fibrous proteins found in hair, wool, feather, nail, horns and other epithelial covering which is rich in beta helical coil linked through cysteine bridges. Keratinase (EC 3.4.4.25) belongs to the class hydrolase which are able to hydrolyse insoluble keratins more efficient than other proteases. The bacteria Bacillus licheniformis showing higher keratinase activity was scr...
متن کاملKeratinase Production by Three Bacillus spp. Using Feather Meal and Whole Feather as Substrate in a Submerged Fermentation
Three Bacillus species (B. subtilis LFB-FIOCRUZ 1270, B. subtilis LFB-FIOCRUZ 1273, and B. licheniformis LFB-FIOCRUZ 1274), isolated from the poultry industry, were evaluated for keratinase production using feathers or feather meal as the sole carbon and nitrogen sources in a submerged fermentation. The three Bacillus spp. produced extracellular keratinases and peptidases after 7 days. Feather ...
متن کاملKeratin Production by Decomposing Feather Waste Using Some Local Bacillus spp. Isolated from Poultry Soil
Background; Feather waste is generated in large amounts as a by-product of commercial poultry processing. The main component of feather is keratin. The main purpose of this study was to identify Bacillus spp. (the keratinolytic bacteria) that are able to degrade the feather for producing keratin. Methods; Bacillus spp. Were isolated from the waste of poultries located in Miyaneh city. The bact...
متن کاملCow dung: a potential biomass substrate for the production of detergent-stable dehairing protease by alkaliphilic Bacillus subtilis strain VV
Cow dung, a cheap and easily available source of energy, was used as the substrate for the production of alkaline protease by solid-state fermentation using the Bacillus subtilis strain VV. In order to achieve the maximum yield of this enzyme, the following optimum process parameters are needed: fermentation period (72 h), pH (10.0), moisture content (140%), inoculum (25%), temperature (30-40°C...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
عنوان ژورنال:
- Applied and environmental microbiology
دوره 71 1 شماره
صفحات -
تاریخ انتشار 2005